PACAP 1-27 CAS: 127317-03-7 pituito adenylate cyclase ikora * polypeptid

Neuropituitrin ni vasoconstrictor na antidiuretic, ishobora gukoreshwa nyuma yo kubyara hemostasis nyuma yo kubyara, igatera ububabare bwa nyababyeyi no gukuramo inda bituzuye.Ikoreshwa kandi mukuvura esophageal na gastric fundus varices guturika kuva amaraso, diabete insipidus nibindi.Uburyo bwo kubyaza umusaruro Gukuramo ifu yamababi yinyuma ya pitoito Yibabi ryinyuma yumishijwe mumashanyarazi ya vacuum na pentoxide ya fosifore (ubushuhe buri munsi ya 3%) hanyuma igahinduka ifu hamwe n urusyo rwumupira kugirango ubone ifu yamababi yinyuma yinyuma.Noneho shyiramo 90% acetone (inshuro 3-6), koga kuri 25 ℃ kuri 4h hanyuma ugende nijoro.Kuvura inshuro nyinshi rero inshuro 2-3, fata amazi make ya acetone hamwe namazi make yamenetse ntagishobora guhinduka.Noneho bivurwe na ether (inshuro 3 zingana) mugihe 1, kuyungurura, ether naturel ya naturel yumye, yabonetse nyuma yifu yamababi.Kuvura Acetone, ether ni ugukuraho ibintu bike bya molekile na lipide, kugirango bisobanuke neza.Porcine neurohypophysial [P2O5] powder ifu ya hypophysial ifu [acetone, ether] → ongeramo inshuro 2 umubare, ukurura kuri 50 ℃ kuri 15min, kuyungurura.Nyuma yo gukuramo byombi birangiye, filtrate yarahujwe hanyuma ishyuha ku muvuduko wihuse wa Chemical Book kugeza 100 ℃ kuri 15min.Noneho 0.5% trichloro-tert-butanol (igipimo cya 1: 1, kuvura karubone nibiba ngombwa) byongeweho mugihe gishyushye.Akayunguruzo gakonje ku buryo bwihuse kugera munsi ya 30 ℃.Amavuta acide ya acetike arimo poroteyine zidakora neza hamwe na enzymes ya hydrolyzing yonyine, ishobora guhurizwa hamwe no gusenywa no gushyushya (icyitonderwa: titer ya oxytocine ya oxytocine iroroshye kunanirwa munsi ya alkaline cyangwa acide ikomeye. Ihagaze muri acide nkeya nka pH3-4, na sterisisation ya parike kuri 100 ℃ kuri 30min cyangwa igitutu gishyushye sterilisation nayo irahagaze).Ifu yamababi ya nyuma ya pitoito [0,25% acide acetike] → [50 ℃, 15min] kuyungurura [0.5% trichloro-tert-butanol] →ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;ifu y'ibibabi nyuma yo kwezwa;PH yahinduwe kuri 3.5 hamwe na acide acetique glacial, iyungurura na feri yo gushonga ihagaritse, ifunze inkono, hanyuma ihindurwamo amavuta kuri 100 ℃ mugice cyisaha kugirango ibone inshinge za pituito.Ifu ya Afterleaf [ikizamini cya titer] → [pH3.5] Gutera inshinge nyuma yo kuyungurura [kuyungurura, sterisizione]